Zebrafish are becoming one of the most used vertebrates in developmental and biomedical research. Fish are commonly killed at the end of an experiment with an overdose of tricaine methanesulfonate (TMS. also known as MS-222). but to date little research has assessed if exposure to this or other agents qualifies as euthanasia (i. https://www.sahmbuffy.com/product-category/lite-plan-ned-emergency-modules/